Treatment

Incidence of EAE

Mean peak clinical score

Suppression of EAE

Incidence

Score

Female PLP-primed T cells

6/6

3.83

 

 

PTIO-treated female PLP-primed T cells

0/6

0.17

100%

95%a

Male PLP-primed T cells

0/6

0.25

100%

93%a

DETA-NONOate-treated male PLP-primed T cells

6/6

3.5

 

 

Splenocytes isolated from male and female PLP-TCR transgenic mice were reprimed with PLP 139-151 in the presence or absence of 50 μM PTIO and 100 μM DETA-NONOate. After 4 d, CD4+ T cells were purified and 5 × 106 PLP-specific T cells were transferred to naïve female SJL/J mice via tail vein. Six recipient mice (n=6) were used in each group. While a clinical score of 1 or more was considered as the incidence of EAE in mice. ap < 0.001 vs female PLP-primed T cells.
Table 2: Regulation of encephalitogenicity of male and female PLP-primed T cells by NO modulators.