Tissue compartment TLR2$
Control Antibiotic Gnotobiotic
Dome epithelial surface 1.75 ± 0.25 1.50 ± 0.29 0.75 ± 0.48*
Villous epithelial surface 1.75 ± 0.25 1.75 ± 0.25 1 ± 0.41*
Villous goblet cells 0.50 ± 0.50 0.33 ± 0.33 0.25 ± 0.25*
Crypt epithelial surface 0.75 ± 0.48 1 ± 0 0.25 ± 0.25
Dome 2.20 ± 0.37 2 ± 0.45 1.40 ± 0.24
Corona 1.80 ± 0.37 1.20 ± 0.49 1.20 ± 0.37
Follicle 1.83 ± 0.17 1.80 ± 0.37 1.50 ± 0.22
Inter follicular area 0.67 ± 0.21 0.80 ± 0.37 0.67 ± 0.21
*Data from 3 or 4 animals only as in some tissues epithelial layer was damaged. $- Statistical differences between the control vs antibiotics, control vs gnotobiotic, and antibiotic vs gnotobiotic groups were calculated and P-value of ≤0.05 was considered significant. No significant differences in various compartments were obtained as P-values between different treatments were ≥0.05.
Table 1a: TLR2 immunohistochemistry. TLR2 immunohistochemistry data.