Tissue compartment TLR9$
Control Antibiotic Gnotobiotic
Dome epithelial surface 1.75 ± 0.25 1.50 ± 0.29 0.50 ± 0.28*
Villous epithelial surface 1.75 ± 0.25 1.50 ± 0.29 0.75 ± 0.25*
Villous goblet cells 0.50 ± 0.50 0 ± 0 0.25 ± 0.25*
Crypt epithelial surface 0.50 ± 0.29 1 ± 0 0 ± 0
Dome 1.60 ± 0.24 1.20 ± 0.20 1 ± 0
Corona 1.20 ± 0.20 0.80 ± 0.37 0.80 ± 0.19
Follicle 1.33 ± 0.21 1.40 ± 0.24 1 ± 0
Inter follicular area 0.67 ± 0.21 0.60 ± 0.24 0.67 ± 0.21
*Data from 3 or 4 animals only as in some tissues epithelial layer was damaged. $- Statistical differences between the control vs antibiotics, control vs gnotobiotic, and antibiotic vs gnotobiotic groups were calculated and P-value of ≤0.05 was considered significant. No significant differences in various compartments were obtained as P-values between different treatments were ≥0.05.
Table 1b: TLR9 immunohistochemistry. TLR9 immunohistochemistry data.