Figure 4: Bcl-2D/A or Bcl-xLD/A inhibits caspase-9-induced release of cytochrome c and Smac. Control H9-iCasp9 cells, or H9-iCasp9 cells expressing either wild type or cleavage-resistant Bcl-2 or Bcl-xL, were treated with 10 nM of CID for the indicated times, followed by isolation of the cytosolic and particulate fractions. Western blotting was performed for the indicated proteins. Membranes were probed with anti-GAPDH or anti-VDAC antibodies to confirm equal loading. Results are representative of three experiments.