Figure 5: CRTH2 inhibitor reduced pro-inflammatory cytokine gene expression in the colon. RNA was isolated from mouse colon at study termination described in Figure 4. Quantitative RT-PCR for various cytokine transcripts was performed using prequalified primers and probes on colon RNA. Results reported as group means ± SEM. Results are representative of at least 3 studies, for experiments shown 8-10 mice were used per treatment group.