Table 2

Cell population compared	Cell surface markers	Health controls (n=18) Median % (Interquartile range)	Sarcoids (n=27) Median % (Interquartile range)	Control versus sarcoidosis group p-value
B cells (% lymphocytes)	CD19⁺	9.79 (4.97)	8.00 (7.10)	0.24
Memory B cells (% B cells)	CD19⁺CD27⁺	28.70 (23.87)	13.50 (16.92)	0.14
Switched memory B cells (% lymphocytes)	CD19⁺CD27⁺IgD^-IgM^-	0.82 (0.80)	0.47 (0.60)	0.03
Switched memory B cells (% B cells)	CD19⁺CD27⁺IgD^-IgM^-	8.75 (8.80)	5.67 (10.66)	0.28
Unswitched memory B cells (% lymphocytes)	CD19⁺CD27⁺IgD⁺IgM⁺	1.30 (0.99)	0.58 (0.57)	0.01
Unswitched memory B cells (% B cells)	CD19⁺CD27⁺IgD⁺IgM⁺	12.41 (15.46)	6.28 (8.43)	0.02
naive-B cells (% lymphocytes)	CD19⁺CD27^-IgD⁺IgM⁺	5.26 (1.88)	4.26 (3.96)	0.62
naive-B cells (% B cells)	CD19⁺CD27^-IgD⁺IgM⁺	59.19 (37.22)	61.96 (25.34)	0.48
CD38low CD21low cells (% B cells)	CD19⁺CD38loCD21lo	2.82 (6.62)	2.32 (3.15)	0.18
Transitional B cells (% B cells)	CD19⁺CD38⁺⁺IgMhi	3.76 (2.74)	2.77 (4.36)	0.37
Plasmablasts (% B cells)	CD19⁺CD38⁺⁺IgM^-	1.05 (1.35)	0.89 (1.24)	0.42
IgD⁺ memory B cells (% B cells)	CD19⁺CD27⁺IgD⁺	18.87 (16.69)	8.38 (7.99)	0.05

Table 2: Statistical analysis on the proportions of peripheral blood B cell populations compared between healthy controls and sarcoidosis patients
All statistical tests were performed in both Excel and Sigma-Stat 3.1. All values are expressed as a percentage of either total lymphocytes determined by FSC/SSC gating or B cells determined by CD19 gating. The Mann-Whitney U test was used for statistical analysis; the median, interquartile range and p-values for each of the groups are shown.
It should be noted that the application of a multiple testing correction (Benjamini- Hochberg) to the analysis resulted in a loss of significance at 0.05 in the IgD⁺ memory B cells (%B cells).