Cell surface markers/phenotype Expressed as Healthy Controls (n=12) Healthy Controls (n=12)   Mean Sarcoids (n=17)   Mean Control versus sarcoid p-value CD3+CD4-CD45RO+CD45RA-  (memory T cytotoxic) lymphocytes 9.09 5.97 0.038 T cells 16.07 10.47 0.028 CD4- cells 43.13 30.07 0.085 CD3+CD4-CD45RA+CCR7+ (naïve T cytotoxic) lymphocytes 8.39 4.26 0.034 T cells 12.79 6.59 0.013 CD4- cells 30.99 18.40 0.017 CD3+CD4+CD28-CD27- (late differentiated T helper) lymphocytes 0.02 0.16 0.016 T cells 0.03 0.36 0.008 CD4+ cells 0.05 1.45 0.013 CD3+CD4-CD28+CD27+ (early differentiated T cytotoxic) lymphocytes 17.82 10.59 <0.001 T cells 28.02 18.24 <0.001 CD4- cells 78.46 55.90 0.008 CD3+CD4-CD28-CD27- (late differentiated T cytotoxic) lymphocytes 1.21 6.14 0.054 T cells 2.08 10.12 0.048* CD4- cells 6.27 28.86 0.006 CD3+CD4-CD25-CD127+ (CD127+ T cytotoxic) lymphocytes 17.43 11.33 <0.001 T cells 26.74 18.46 <0.001 CD4- cells 72.84 52.29 0.002 CD3+CD4-CD45RA+CD31- (CD31- naïve T cytotoxic) lymphocytes 0.83 2.05 0.048* T cells 1.29 3.42 0.016 CD4- cells 2.92 8.16 0.003

Table 3: Statistical analysis of the peripheral blood T cell populations in healthy controls and sarcoidosis patients All statistical tests were performed in both Excel and SigmaStat 3.1. All values expressed as a percentage of either total lymphocytes determined by FSC/SSC gating, T cells as determined by CD3 staining and as % of either the CD3+CD4+ or CD3+CD4- populations. Lymphocyte sub-populations were compared between groups and either t-test or Mann-Whitney U tests were performed depending on the results of the Kolmogorov-Smirnov normality test. *These values did not retain significance at a critical value cut-off of 0.05 when a Benjamini-Hochberg multiple testing correction was applied. All other significant values highlighted in bold retained significance at this level after multiple testing corrections were applied.