A. Following addition of antibodies to the media, Atto-550-NHS labeled antihnRNPA1 and control antibodies (red) were found to penetrate neuronal cells.
B. Quantification of antibodies found inside the cells. Five images were used per treatment. Antibody penetrated cells were divided by the total number of cells to give a percentage of antibody penetration. (Cells with antibodies per image/Total cells per image)×100=Penetration Percentage.
Figure 1: Localization of antibodies by immunocytochemistry in SK-N-SH cells.