Figure 7: Antibodies cause redistribution of endogenous hnRNP A1. A. Quantification of endogenous hnRNPA1 redistribution in comparison to untouched cells and cells with control IgG. Nuclear to cytoplasmic fluorescence ratio calculated using densitometric means, n=5. B. Quantification of endogenous hnRNP A1. Nuclear and cytoplasmic fractions were collected from untouched, IgG and hnRNPA1 treated SK-N-SH cells. Equal amount of each fraction per condition was loaded onto 10% SDS gel. The gel was transferred onto nitrocellulose membrane and probed with anti-hnRNPA1 antibody (Abcam). Band quantitation was performed in Image J program using gel analysis method.