Figure 4: Effects of Gag p24 and LTB on antigen presentation of an unrelated antigen, OVA.
Splenic cells were isolated from normal mice and incubated in the absence of antigen (medium) or with OVA (0.5 mg/ml); OVA (0.5 mg/ml)+gag p24 (10, 20 and 40 μg/ml); OVA (0.5 mg/ml)+gag p24 (10, 20 and 40 μg/ml)+30 μg/ml LTB; or OVA (0.5 mg/ml)+LTB (30 μg/ml), for 6 hrs at 37°C to allow uptake. DO.11.10 T cells hybridoma were pre-labeled with CFSE and added to the culture above. The cells were then incubated for 3 days and analyzed by flow cytometry. In (A), the dot plot analysis shows 2 populations of cells of lower and higher FSC representing splenic cells and DO.11.10 respectively. Dead cells of very low FSC and high SSC were gated out. CFSE-labeled DO.11.10 cells are also shown in histogram after gating on the population above. The other histograms show number of DO.11.10 cells in the presence of various treatments as indicated. In (B), the histograms show proliferation profile of those DO.11.10 T cells in cultures incubated with OVA, OVA+gag p24 (20 μg/ml) and OVA+gag p24 (20 μg/ml)+LTB.