Figure 3: Profile of IgG subclass in sera from experimental plague infected mice. [A]. Sera samples collected on day 22 and were analyzed for presence of YopH-specific IgG1, IgG2a IgG2b and IgG3; [B] V antigen-specific IgG1, IgG2a IgG2b and IgG3; [C] YopE antigen-specific IgG1, IgG2a IgG2b and IgG3; [D] F1- specific IgG1, IgG2a IgG2b and IgG3 subclass antibodies by ELISA. The statistical comparisons were performed using Sigma Stat 4 Software (Aspire Software International, USA). *P<0.05; **P< 0.01. ***P<0.001. Analysis was done by t-test.