Figure 4: TLRs, CD14, MyD88, NF-κB, TNF-α, IL-1β, MAPKp38 expression in a time dependent manner in peritoneal macrophages of experimental plague infected mice. [A]. Fold change expression of TLR1 to TLR5. [B] Fold change expression in TLR6 to TLR9, CD14. [C] Fold change expression in MyD88, NF-κB, TNF-α, IL-1β, MAPKp38. The gene expression level was evaluated by qRT-PCR and normalized to the expression of the reference gene glyceraldehyde-3-phosphate-dehydrogenase. Fold change expression was analyzed and represented in terms of normalized fold expression with ± 1 standard deviation using CFX ManagerTM software (BioRad, USA).