| Gene |
Oligos |
Restriction sites |
Amplicon size (bp) |
Cloning vector |
NCBI accession No |
| ypkA |
For-5&rs’-CATATGAAAAGCGTGAAAATCATGG-3’
Rev-5’-GTCGACTCACATCCATTCCCGCTC-3’ |
Nde I
Sal I |
2199 |
pET28a+ |
AF074612.1 |
| yopH |
For-5’-ATACCATGGGCATGAACTTATCATTAAGCGATCTTCATC-3’
Rev-5’-ATACTCGAGGCTATTTAATAATGGTCGCCCTTG-3’ |
Nco I
Xho I |
1407 |
pET28a+ |
AF053946.1 |
| yopM |
For-5’-GGTACCATGTTCATAAATCCAAGAAATGTATCTAA-3’
Rev-5’-GTCGACCTACTCAAATACATCATCTTCAAGTTTGT-3’ |
Kpn I
Sal I |
1230 |
pET30a+ |
AF053946.1 |
| lcrV |
For-5’-CATATGATTAGAGCCTACGAACAAAAC-3’
Rev-5’-GTCGACTCATTTACCAGACGTGTCATCTAG-3’ |
Nde I
Sal I |
981 |
pET28a+ |
NC 003131.1 |
| pla |
For-5’-ATACCATGGGCATGAAGAAAAGTTCTATTGTGGCAAC-3’
Rev-5’-ATACTCGAGGAAGCGATATTGCAGACCCG-3’ |
Nco I
Xho I |
939 |
pET28a+ |
HM807367.1 |
| yopN |
For- 5’-CATATGACGACGCTTCATAACCTATC-3’
Rev- 5’-GTCGACTCAGAAAGGTCGTACGCCAT-3’ |
Nde I
Sal I |
882 |
pET28a+ |
AF053946.1 |
| yopJ |
For-5’-CATATGATCGGACCAATATCACAAAT-3’
Rev-5’-GTCGACTTATACTTTGAGAAGTGTTTTATATTCAGC-3’ |
Nde I
Sal I |
867 |
pET28a+ |
AF074612.1 |
| yopE |
For-5’-CATATGAAAATATCATCATTTATTTCTACATC-3’
Rev-5’-GTCGACTCACATCAATGACAGTAATTTCTG-3’ |
Nde I
Sal I |
660 |
pET28a+ |
NC_005813.1 |
| yopK |
For-5’-CATATGTTTATTAAAGATACTTATAACATGCGTG-3’
Rev-5’-GTCGACTCATCCCATAATACATTCTTGATCG-3’ |
Nde I
Sal I |
549 |
pET28a+ |
AF053946.1 |
| Caf1 |
For-5’-ATACCATGGGCATGAAAAAAATCAGTTCCGTTATCG-3’
Rev-5’- ATACTCGAGTTGGTTAGATACGGTTACGGTTACAG-3’ |
Nco I
Xho I |
513 |
pET28a+ |
AF074611.1 |
| pH6 |
For-5’-ATACCATGGGCATGAAAATGAAATGTTTTGCG-3’
Rev-5’-ATACTCGAGAAATACATACTCTTCAACACGCC-3’ |
Nco I
Xho I |
477 |
pET28a+ |
NC_010159.1 |
