Oligos Name Forward primer Reverse primer PCR Product (bp) Accession number
TLR1 5’-ATGTGAGCTGAGGGACTTTG-3’ 5’-GGATAGTGGAGACATGTGGAAG-3’ 141 AF316985
TLR2 5’-ACTGTGTTCGTGCTTTCTGAG-3’ 5’-ATGGCTTTCCTCTCAATGG-3’ 139 AF124741
TLR3 5’-ATAAATGAGGACTTGCTGGAGG-3’ 5’-TCAGGAAATTAACGGGACCAC-3’ 114 AF355152
TLR4 5’-GAGGACTGGGTGAGAAATGAG-3’ 5’-GTAGTGAAGGCAGAGGTGAAAG-3’ 74 AF185285
TLR5 5’-AAGACTGCGATGAAGAGGAAG-3’ 5’-CTGGATGGTCTTATAGCACAGG-3’ 145 DQ414410
TLR6 5’-GTCAAGAACATAGGCTGGGTAG-3’ 5’-GCAGAACAGTATCACAGGACAG-3’ 138 AF314636
TLR7 5’-CACAGGCTCACCCATACTTC-3’ 5’-ACTTCAGGTACCAAGGCATG-3’ 141 AF334942
TLR8 5’-CTGGAGACACTGCTACTGAG-3’ 5’-TCATCTTGGTTTGCAGGGAG-3’ 141 AY035890
TLR9 5’-CTCCTTGATCTCCAACCGTATC-3’ 5’-ACAGTTCCACTTGAGGTTCAG-3’ 90 AF348140
CD14 5’-CTTTTCTCGGAGCCTATCTGG-3’ 5’-CAACTTTCCTCGTCTAGCTCG-3’ 138 BC057889
MyD88 5’-TCGAGTTTGTGCAGGAGATG-3’ 5’-GCGACACCTTTTCTCAATTAGC-3’ 136 U84409
NF-κB 5’-CACGGTTATGGGCAGGAAG-3’ 5’-GCACCTTTGGATTCGCTTTG-3’ 134 AY521462
TNF-α 5’-TTGCTCTGTGAAGGGAATGG-3’ 5’-CCTGAGCCATAATCCCCTTTC-3’ 140 NM013693
MAPKp38 5’-AGATGCTCGTTTTGGACTCAG-3’ 5’-GGTCGTGGTACTGAGCAAAG-3’ 80 AF195850
IL-1β 5’-ACGGACCCCAAAAGATGAAG-3’ 5’-TTCTCCACAGCCACAATGAG-3’ 138 BC011437
GAPDH 5’-CCCCAGCAAGGACACTGAGCAAG-3’ 5’-TCTGGGATGGAAATTGTGAGGGAGA-3’ 86 NT187045
Table 2: List of primers and the target sequences used for Quantitative real time PCR for analysis of TLRs and other cytokines in mice infected with Y. pestis.