Figure 1: Calpain concentration dependent T cell proliferation. Proliferation of MBP Ac1-11-specific T cells either in the presence of no antigenic peptide, the native MBP Ac1-11 (4K) peptide, or a strong agonist APL MBP Ac1-11 with an alanine substitution at position 4 (4A). Cells were incubated in the presence of several concentration of the calpain inhibitor calpeptin. A. The raw 3H-thymidine concentration. B. % 3H-Thymidine relative to the vehicle group set at 100. Mean ± SEM (n=3). *, p<0.05 vs. control; †, p<0.05 vs. vehicle group. C. MBP Ac1-11-specific cells were incubated in the presence or absence of the native MBP peptide (4K) and shown over several doses of calpeptin. Mean ± SEM (n = 4). *, p<0.05 vs. unstimulated control; †, p<0.05 vs. stimulated group.