Figure 2: The combination of (LPS+IFN-γ) upregulates the iNOS protein expression in astrocytes and microglia, and down-regulates the expression of MOG and PLP in oligodendrocytes: Human mixed glial cells were treated with the combination of LPS (1 μg/ml) and IFN-γ (6.25 mU/ml) under serum free conditions. After 24 h, cells were immunostained with iNOS & Iba1 (A), iNOS & GFAP (B), PLP & GFAP (C), and MOG & PLP (D). Scale bars represent 10 μM.