Figure 1: Effects of 50 μM docosahexaenoic (DHA), eicosapentaenoic (EPA), stearic (SA), and palmitic (PA) acids or 25 μM oleic (OA) and linoleic (LA) acids on IL-2-induced JAK1 phosphorylation. Lymphocytes were incubated with 5 μg/mL ConA for 24 h. Afterwards, lymphocytes were washed with PBS and cultured with the different fatty acids in the presence or absence of IL-2 (30 ng/mL) for 1 h. Total proteins were extracted from lymphocytes for western-blotting analysis. Blots were analyzed by densitometry and the results normalized to their respective controls, which were set to 100% in each experiment. The values are presented as the means ± SEM. #p<0.05 and ##p<0.01 for comparison with the control in the absence IL-2; **p<0.01 and ***p<0.001 for comparison with the control treated with IL-2.