Figure 7: Effects of 50 μM DHA, EPA, SA, and PA or 25 μM OA and LA on CD25-alpha lymphocyte surface expression. Lymphocytes were stimulated with 5 μg/mL ConA for 24 h, washed, and incubated with 30 ng/mL IL-2 for 1 h. Cells were pelleted and labeled with an FITC-conjugated anti-CD25 antibody and analyzed using flow cytometry. Negative control cells were incubated with a labeled nonreactive control antibody. Histograms from 10,000 events were analyzed. Fluorescence was measured using the FL1-H channel (green fluorescence, 530/30 nm). A) Representative histograms obtained using flow cytometry. B) Percentage of cells expressing CD25. The values are presented as the means ± SEM of three determinations from four experiments. *p<0.05 for comparison between fatty acid treatments vs. the control (in the absence of FAs and IL-2); ●p<0.05 for comparison between fatty acid treatments vs. the control treated with IL-2.