Figure 8: Effects of 25 μM OA and LA on IL2-induced lymphocyte proliferation (A) and PKC-ζ phosphorylation (B) in the presence of wortmannin (W). (A) Lymphocytes were stimulated with ConA for 24 h, washed, and incubated with 100 nM of wortmannin, 30 ng/mL IL2 and 25 μM OA and LA for 30 h and after [2-14C]-thymidine (1 μCi per mL) was added and cells incubated for 18 h. Data are expressed as counts per minute (cpm) and presented as mean ± S.E.M. of three determinations from three experiments. (B) Lymphocytes were stimulated with ConA for 24 h, washed, and incubated with 100 nM of wortmannin, 30 ng/mL IL-2 and 25 μM OA and LA for 60 minutes. Afterwards, protein kinase C (PKC)-ζ phosphorylation was evaluated by western-blotting. After densitometry analysis, the data were normalized to their respective controls, which were set to a value of 100% for each experiment. #p<0.05 for comparison between fatty acid treatments vs. the control (in the absence of FAs and IL-2); **p<0.05 for comparison between fatty acid treatments vs. the control treated with IL-2; ♦p<0.05 for comparison between fatty acid treatments in the presence of wortmannin vs. fatty acid treatments in the presence of IL-2.