Figure 2: (A) Kainic acid-induced alterations in the expression of various cytokine (IL-10, IL-1, IL-6, TNF-β, INF-γ) mRNA from splenic lymphocytes isolated from mouse spleen and their attenuation by melatonin treatment using real time PCR. (a) Normal control, (b) Kainic acid control (1 mM), (c) Melatonin (1 mM), (d) Kainic acid (1 mM)+Melatonin (0.25 mM), (e) Kainic acid (1 mM)+Melatonin (0.5 mM), (f) Kainic acid (1 mM)+Melatonin (1 mM); IL10-interleukin 10; IL6- interleukin 6; TNF-β: tumour necrosis factor beta; INFγ: interferon gamma. Values are mean ± S.D; *P<0.005(compared to respective normal control); #P<0.05 (compared to Kainic acid control) and $P<0.005 (compared to Kainic acid control). n=3 in each group. (B) Kainic acid-induced alterations in the expression of various cytokine (IL-10, IL-1, IL-6, TNF-β, INF-γ) mRNA from enriched T lymphocytes isolated from mouse spleen and their attenuation by melatonin treatment using real time PCR. (a) Normal control, (b) Kainic acid control (1 mM), (c) Melatonin (1 mM), (d) Kainic acid (1 mM)+Melatonin (0.25 mM), (e) Kainic acid (1 mM)+Melatonin (0.5 mM), (f) Kainic acid (1 mM)+Melatonin (1 mM); IL10-interleukin 10; IL6-interleukin 6; TNF-β: tumour necrosis factor beta; INFγ: interferon gamma. Values are mean ± S.D; *P<0.005 (compared to respective normal control); #P<0.05 (compared to Kainic acid control) and $P<0.005 (compared to Kainic acid control)). n=3 in each group.