Figure 2: Optimization of imiquimod induced skin inflammation. Imiquimod or vehicle cream was applied to the shaved back and one ear of the BALB/c mice with different frequencies as depicted in the figure. (A) Ear thickness was measured before imiquimod application and at multiple time points during the 5 day study. (B) At the study termination, mice were sacrificed and ears were harvested for H&E stain. Representative images are shown. (C) Two to three days post imiquimod application, mice were sacrificed and ears were harvested. Real time RT-PCR on RNA isolated from individual mouse ears from each group for indicated cytokines was determined. Results reported as mean ± SEM. All data represent the mean of at least two independent experiments n=6 for each group.