Figure 4: Expression of the adrenergic receptor β2-AR in control and psoriatic PBMC. (A) Quantitative RT-PCR was performed on total mRNA extract from control and psoriatic PBMC, as detailed in Materials and Methods; each sample was assayed in duplicate and the averaged values used for the box-plot representation. (B) Upper panel: representative Western blotting on total cell protein extract (40 μg/lane) from three control and three psoriatic PBMC. Lower panel: box-plot representation of the densitometric analysis of the immunodetected β2-AR band normalized to that of β-actin. White boxes, control; red boxes, psoriatic. NS, non-statistically significant (i.e. P>0.05).