Figure 5: Expression of transcription factors involved in mitochondrial biogenesis and of subunits of complex I in control and psoriatic PBMC. Quantitative RT-PCR was performed on total mRNA extract from control and psoriatic PBMC, as detailed in Materials and Methods; each sample was assayed in duplicate and the averaged values used for the box-plot representation. (A) Transcript levels of nuclear respiratory factor 1 (NRF1), nuclear respiratory factor 2 (NRF2) and mitochondrial transcription factor A (TFAM). (B) Transcript levels of the complex I subunits ND5 (mitochondrial DNA encoded) and NDUFS8 and GRIM19 (nuclear DNA encoded). White boxes, control; red boxes, psoriatic. Statistically significant differences are shown when P<0.05; NS, non-statistically significant (i.e. P>0.05).