Figure 1: Gating strategy of regulatory T-cells (CD3+CD4+CD25Bright FoxP3+). (A) Shows the live lymphocyte gate, (B) Shows the CD3+CD4+lymphocytes, (C) Shows FoxP3 isotype and the Fluorescence minus one (FMO) for CD25, and (D) Shows the Treg (CD3+CD4+CD25Bright FoxP3+ T cells).