Figure 3: Glaucoma risk factors primarily induce astrocyte hypotrophy, while glaucoma induces greater variability in astrocyte size. (A) Quantification of the mean soma area of astrocytes (μm2; y-axis) across all images and samples reveals that the soma of astrocytes in aged C57 and young DBA/2 retina are smaller than that of young C57 and aged DBA/2 retina. Error bars indicate standard deviation and asterisks indicate p<0.05. (B) Histogram function of soma area for young C57 (gray) and aged C57 (black) retina plotted as percent population (y-axis) versus soma area (μm2; x-axis). The histogram functions reveal a reduction in the distribution spread and left shift towards smaller soma areas in aged C57, as compared to young C57 retina. (C) Histogram function of soma area for young DBA/2 (gray) and aged DBA/2 (black) retina plotted as percent population (y-axis) versus soma area (μm2; x-axis). The histogram functions for young and aged DBA/2 retina reveal an increase in the distribution spread, as compared to young C57 (B) retina. Aged DBA/2 retina exhibits the largest spread that lacks a primary peak, while young DBA/2 retina exhibits primary peaks that are shifted towards smaller soma areas.