**Figure 7:** Density, hypertrophy and GFAP expression for spatially-coincident astrocytes are reliably predictable in aged C57 and aged DBA/2 retina. (A and B) Contour
plot depicting the relationships between mean GFAP intensity/cell (color bar; arbitrary units), astrocyte density (x-axis; astrocytes/mm^{2}) and soma area (y-axis; μm^{2})
in aged C57 (A) and aged DBA/2 (B) retina. (C) Regression graph of the negative correlation between astrocyte density and mean GFAP expression/cell in aged C57
retina plotted as astrocytes/mm^{2} (x-axis) versus arbitrary units of intensity (y-axis). (D) Regression graph of the negative correlation between astrocyte density and
mean GFAP expression/cell in aged DBA/2 retina plotted as astrocytes/mm^{2} (x-axis) versus arbitrary units of intensity (y-axis). (E) Regression graph of the negative
correlation between astrocyte density and soma area in aged C57 retina plotted as astrocytes/mm^{2} (x-axis) versus soma area (μm^{2}; y-axis). (F) Regression graph
of the negative correlation between astrocyte density and soma area in aged DBA/2 retina plotted as astrocytes/mm^{2} (x-axis) versus soma area (μm^{2}; y-axis). (G)
Regression graph of the positive correlation between soma area and mean GFAP intensity/cell in aged C57 retina plotted as soma area (μm^{2}; sxaxis) versus arbitrary
units of intensity (y-axis). (H) Regression graph of the positive correlation between soma area and mean GFAP intensity/cell in aged DBA/2 retina plotted as soma area
(μm^{2}; sxaxis) versus arbitrary units of intensity (y-axis). For all graphs, solid lines indicate regression line based on r^{2} value. Dashed lines indicate the 95% confidence
interval. Dotted lines indicate predicted values based on r^{2} and 95% confidence. |