Figure 14: Percent coverage of retinal area by astrocytes is a strong predictor of RGC health in glaucomatous retina. (A) Contour plot depicting the spatial relationships between percent coverage of retinal area by astrocytes (color bar; % coverage), CTB+ RGC density (x-axis; CTB+ RGCs/mm2) and total CTB intensity (y-axis; arbitrary units) in aged DBA/2 retina. (B) Contour plot depicting the spatial relationships between total GFAP intensity (color bar; GFAP (AU)), CTB+ RGC density (x-axis; CTB+ RGCs/mm2) and total CTB intensity (y-axis; arbitrary units) in aged DBA/2 retina. (C) Regression graph of the positive correlation between CTB+ RGC density and percent coverage of retinal area by astrocytes in aged DBA/2 retina plotted as CTB+ RGCs/mm2 (x-axis) versus % GFAP coverage (y-axis). Solid lines indicate regression line based on r2 value. Dashed lines indicate the 95% confidence interval. Dotted lines indicate predicted values based on r2 and 95% confidence. (D) Solid lines indicate regression line based on r2 value. Dashed lines indicate the 95% confidence interval. Dotted lines indicate predicted values based on r2 and 95% confidence. Regression graph of the positive correlation between percent coverage of retinal area by astrocytes and total GFAP intensity in aged DBA/2 retina plotted as % GFAP coverage (x-axis) versus arbitrary units of GFAP intensity (y-axis).