Figure 6: LPS homologous tolerance induction in patients with FMF and ND. Monocytes were pretreated with 100 ng/ml LPS for 18 h, washed with LPS-free PBS and cultured for an additional 4 h in the presence of 1 μg/ml LPS. The production of TNF-α (A), IL-10 (B) and IFN-γ (C) was subsequently assayed by ELISA. All data represent means ± SEM (error bars) and are significantly different comparing FMF with ND (*Pt or Pw<0.05, ** Pt<0.002) or comparing pretreatment with LPS re-stimulation pairs (# Pp<0.05).