Figure 3: Fluorescence homogeneous detection of monoclonal autoantibodies (dsDNA-mAb32 and dsDNA-mAb33) compared to control proteins (BSA, IgG1 and IgG3) in a medium salt buffer (110 mM[Na]+) at 19°C using 500 nM probes [10]. Sequences of the sensor molecules are listed in Table 1. Incubation was performed by adding 0.5×105 IU of the target autoantibody to a solution of corresponding nucleic acid complex. As a reference, the BSA, IgG1 or IgG3 stock solution was used in similar incubation reactions with the probes. Incubation was performed at 37°C for 3 h. Upon cooling to ambient temperature over 2-4 h, the resulting solutions were analysed by fluorescence spectroscopy using excitation wavelength 500 nm and monitoring fluorescence at 530 nm.