Probe# |
Sequence, 5’→3’ |
Tm/ΔTm, °CTarget |
DNA |
RNA |
DNAref |
d(TGCACTCTATGTCTGTATCAT) |
59.0 |
60.5 |
P1 |
d(TGCAC(1)CTATGTCTGTA(1)CAT) |
63.0/+4.0 |
68.0/+7.5 |
P2 |
d(TGCAC(1)CTATG(1)CTGTA(1)CAT) |
65.0/+6.0 |
71.0/+10.5 |
P3 |
d(TGCAC(6)CTATGTCTGTA(6)CAT) |
58.0/-1.0 |
64.0/+3.5 |
P4 |
d(TGCAC(6)CTATG(6)CTGTA(6)CAT) |
58.0/-1.0 |
66.0/+5.5 |
P5 |
d(TGCAC(7)CTATGTCTGTA(7)CAT) |
63.0/+4.0 |
65.0/+4.5 |
P6 |
d(TGCAC(7)CTATG(7)CTGTA(7)CAT) |
65.0/+6.0 |
66.5/+6.0 |
P7 |
d(TGCAC(9)CTATGTCTGTA(9)CAT) |
67.0/+8.0 |
67.0/+6.5 |
P8 |
d(TGCAC(9)CTATG(9)CTGTA(9)CAT) |
72.0/+13.0 |
70.0/+9.5 |