Biotinylated Antibodies Ligands used Dilution factor Serum Antigen-coated Wells CA215 (S+) Ib first purification CIG (S+)b first purification CA215 (S+) IIc second purification   ΔOD405nm (relative reading) CA215       1:50 1.107 ± 0.261b 1.347 ± 0.180 0.312 ± 0.030d 1:100 1.175 ± 0.157 1.345 ± 0.092 0.238 ± 0.060 1:200 0.704 ± 0.161 0.917 ± 0.151 0.095 ± 0.005 CIgG       1:50 1.098 ± 0.172 1.204 ± 0.121   1:100 0.611 ± 0.048 0.742 ± 0.060   1:200 0.317 ± 0.061 0.416 ± 0.122

Table 1: Enzyme immunobinding assay to reveal specific binding between well-coated purified serum antigen, CA215(S+) or CIgG(S+) and CA215 or CIgGa.