Figure 3: Endothelial surface activation marker expression induced by SCLS sera. HUVECs were incubated with 10% (vol/vol) test sera for 4 hours (A-B) or 24 hours (C) at 37°C. (A) Surface expression of E-selectin, VCAM-1 and ICAM-1 was evaluated by flow cytometry. The dotted lines represent adhesion molecule expression induced by TNFα (1.25 ng/ml). (B) Paired comparisons of E-selectin, VCAM-1 and ICAM-1 expression induced by basal and episodic sera from each individual subject. (C) CXCL10 in HUVEC culture supernatants was measured by ELISA.