Gene target Forward sequence Reverse sequence Amplicon (bp) HPRT TGGAGTCCTATTGACATCGCCAGT AGTGCCTCTTTGCTGCTTTCACAC 197 IL-1β AACAGGCTGCTCTGGGATTCTCTT ATTTCACTGGCGAGCTCAGGTACT 92 IL-6 AAATTCGGTACATCCTCGACGGCA AGTGCCTCTTTGCTGCTTTCACAC 88 TNF TGGGCAGGTCTACTTTGGGATCAT TTTGAGCCAGAAGAGGTTGAGGGT 128 IFN-γ ACTAGGCAGCCAACCTAAGCAAGA CATCAGGGTCACCTGACACATTCA 184 IL-1Ra AATCCATGGAGGGAAGATGTGCCT TGTCCTGCTTTCTGTTCTCGCTCA 110 IL-10 TCCTTGCTGGAGGACTTTAAGGGT TGTCTGGGTCTTGGTTCTCAGCTT 109 IL-8 CTTGGCAGCCTTCCTGATTT GGGTGGAAAGGTTTGGAGTATG 111 C5aR AGACATCCTGGCCTTGGTCATCTT TACCGCCAAGTTGAGGAACCAGAT 133

Table 1: Primer sequences and amplicon sizes of housekeeping (HPRT) and target genes (IL-1β, IL-6, TNF, IFN-γ, IL-1Ra IL-10, IL-8 and IL-8) expressed in humans.