Figure 2

Figure 2: NK cell degranulation was measured by the expression of CD107a and CD107b in unstimulated and stimulated NK cells. Comparison of the CFS/ME and control group NK cell degranulation revealed no significant differences in the unstimulated (A) and K562 stimulated cells (B), PMA/I stimulated cells (C) or PHA stimulated cells (D). Significantly increased amounts of CD107a and CD107b were observed when the CD56^brightCD16^-/dim NK cells were compared to the CD56^dimCD16⁺ NK cells stimulated with K562 cells (B), PMA/I (C) and PHA (D) in both the CFS/ME and control groups. CD56^dimCD16^- NK cells stimulated with K562 cells also had a significant increase in CD107a and CD107b expression in comparison to CD56^dimCD16⁺ NK cells from both groups (B). In the PHA stimulated cells, CD56^dimCD16^- NK cells from the control group also expressed significantly increased amounts in comparison to CD56^dimCD16⁺ NK cells (D). Data is presented as mean ± standard error of the mean (*P<0.05 and ****P<0.0001).