Figure 1: Experimental design and purity isolated cells. (A) Monocytes isolated from the blood of healthy donors were differentiated into macrophages by 7-day culture with M-CSF, and the resulting macrophages were cultured for 18 hours with polarizing factors or medium and then for three days with or without the probiotic VSL#3. (B-D) PBMC and monocyte samples were taken on day 0 after Ficoll-Paque centrifugation and magnetic bead purification, respectively, while the macrophage sample was taken from adhered cells on day 7. Cells were stained with anti- CD3-PerCP and anti-CD14-FITC antibodies and analyzed on a FACSAria flow cytometer. Representative graphs are shown.