Figure 3: Co-culture with apoptotic T cells modulates protein expression of IL-23 and TGF-β in DCs. Spleen cells were isolated from C57BL/6J mice and incubated with CD11c antibody. CD11c+ cells (DCs) were sorted using flow cytometry. DCs were incubated with apoptotic (think line) or fresh T lymphocytes (thin dash line) for 24 hrs at 37°C. Cells were stained by anti-mouse CD11c antibody. CD11c+ cells were gated using flow cytometry. Expression of IL-23 (A) and TGF-β (B) in CD11c+ cells is indicated. Isotype control is demonstrated by shading. Error bars shown in this figure represent mean and SD of triplicate determinations of MFI for IL-23 and TGF-β expression in CD11c+ DCs in three independent experiments (n=3, t test, *P< 0.005).