Figure 5

Figure 5: Tolerogenic DCs induced by apoptotic T cells modulate expression of signal molecules on CD4⁺ CD25⁺ regulatory T cells. CD11c⁺ DCs were isolated from C57 BL/6J mice and sorted using flow cytometry. CD11c⁺ DCs were incubated with apoptotic or fresh T cells overnight at 37°C. MOG-specific CD4⁺ CD25- T cells were isolated from 2D2 transgenic mice. CD4⁺CD25- T cells were co-cultured with 0.1 μM MOG peptide-pulsed CD11c⁺ DCs treated with apoptotic (thick line) or fresh T cells (thin line) as a control, and an assay of flow cytometry was carried out. CD4⁺CD25⁺ cells were gated. Expression of GARP (A), CD44 (B), CD62L (C), CD152 (D), CCR6 (E) and CCR7 (F) is indicated. Isotype controls are also shown by shading. Error bars represent mean and SD of triplicate determinations of MFI of expression of signal molecules on CD4⁺ CD25⁺ regulatory T cells in three independent experiments (n=3, t test, *P< 0.05).