Figure 3: Increased levels of Th17 cells in cervical lymph nodes of mice with premalignant oral lesions. Cervical lymph nodes were collected from control mice or mice with premalignant oral lesions or HNSCC (pooled from 5 mice per group). The lymph nodes were dissociated and cells were incubated for 4 hours with PMA, ionomycin and brefeldin A. Cells were then surface immunostained for CD4, permeabilized, and immunostained for intracellular IL17. Shown are representative scattergrams from 4 separate studies.