Figure 4: Media conditioned by premalignant oral lesions stimulates IL17 production. Cells from enzymatically dissociated normal tongue epithelium, premalignant oral lesions and HNSCC were cultured for 36 hours. Media conditioned by these cells were then added to normal spleen cells (top graph) or Th17-skewed spleen cells (bottom graph). Levels of secreted IL17 were measured by CBA. *p<0.05; **p<0.01.