Figure 1: Comparison of gel mobility and glycoform ratio of PKresistant PrPSc from iCJD and sCJD. (A) Brain homogenates from eight iCJD cases and five sCJD cases were treated with PK (50 µg/ml) prior to Western blotting with 3F4. PRNP analysis shows that cases 1 through 5 are of homozygous MM, cases 6 and 7 heterozygous MV and case 8 homozygous VV polymorphism at residue 129. Cases 1 through 6 share with sCJDMM1 and case 7 with sCJDMV2 the gel mobility and glycoform ratio. The gel mobility and glycoform ratio of PKresistant PrPSc from iCJD matched with those of sCJD. (B) Quantitative analysis of glycoform ratios of PrPSc from iCJDMM (n = 5) and sCJDMM (n = 3). There were no significant differences in glycoform ratios of PrPSc between iCJD and sCJD.