Figure 4: Cytokine multiplex assay: Ex-vivo stimulation of PBMCs with extracts from wings and legs, purified extractsfromgel chromatography, DEAE chromatography, positive controls (CEF and CD3/CD28) or individual negative controls (Total extract: lysis buffer, Gel: buffer B or DEAE: PBS) was screened by multiplex cytokine bead assay in duplicatesof a single experiment. Different levels of fluorescence intensity (FI) of IFN-γ (A), TNF-α (B), IL-2 (C) and IL-10 (D) are shown.