Mass in kDa Arginine kinase Before chromatography After chromatography 160 Mascot Score 200 404 Peptide Matches 5 14 130 Mascot Score 193 414 Peptide Matches 5 13 75 Mascot Score 184 393 Peptide Matches 6 11 65 Mascot Score 383 356 Peptide Matches 11 9

Table 2: Marked increase in Mascot scores and the number of peptide matches to arginine kinase in gel-chromatography-enriched cricket wings/legs proteins.