Mass in kDa Protein Mascot Score Peptide Matches
Before chromatography After chromatography Before chromatography After chromatography
160 Myosin heavy chain 3852 605 98 17
Sar/endoplasmic reticulum calcium ATPase 972 0 25 0
Actin 748 0 25 0
Paramyosin 316 0 8 0
Tubulin alpha 287 267 7 7
ADP/ATP translocase 270 0 6 0
ATP synthase beta subunit 249 0 5 0
Adenine nucleotide translocase 221 0 5 0
130 Myosin heavy chain 2853 1337 69 32
Sar/endoplasmic reticulum calcium ATPase 1200 0 37 0
Paramyosin 779 0 20 0
Actin 544 0 16 0
ADP/ATP translocase 169 0 5 0
Mitochondrial ATP synthase alpha-subunit* 162 216 3 5
75 Myosin heavy chain 1285 965 24 18
Actin 565 0 16 0
Sar/endoplasmic reticulum calciumATPase 565 0 11 0
Paramyosin 186 0 4 0
Sodium/potassium-transporting ATPase alpha chain 160 0 4 0
Tropomyosin** 154 151 3 3
65 Myosin heavy chain 1200 971 27 14
ATP synthase subunit alpha 938 0 24 0
Tubulin beta 834 181 32 6
Actin 830 0 32 0
Tubulin alpha 493 0 12 0
Troponin 476 0 18 0
ATP synthase subunit beta 316 0 7 0
*enriched after chromatography; **no change after chromatography
Table 3: Total depletion or substantial decrease in Mascot scores and the number of peptide matches following gel-chromatography to proteins initially detected in immuno-reactive crude protein extracts.