Figure 5: Nilotinib and bosutinib alter microglia morphology and reduce the number of astrocyte and dendritic cells. Coronal 20 μm thick brain sections show IBA-1 and nuclear DAPI staining of microglia in the striatum of A) WT mice, B) A53T mice treated with DMSO, insert is higher magnification, C) A53T mice treated with bosutinib, insert is higher magnification, and D) A53T mice treated with nilotinib, insert is higher magnification. E) histograms represent stereological quantification. Coronal 20 μm thick brain sections show GFAP and nuclear DAPI staining of astrocytes in the striatum of F) WT mice, G) A53T mice treated with DMSO, H) A53T mice treated with bosutinib, and I) A53T mice treated with nilotinib. J) histograms represent stereological quantification. Dendritic cells stained with CD11b and nuclear DAPI labeling in the striatum of K) WT mice, L) A53T mice treated with DMSO, M) A53T mice treated with bosutinib, and N) A53T mice treated with nilotinib. O) histograms represent stereological quantification. n=5 for each strain at each time point. n=4, ANOVA, Neuman Keuls, Mean ±, * indicates significantly different than WT with p<0.05, **p<0.01.