Figure 3: TLR agonist stimulations increased the secretion of Proinflammatory Cytokines (TNF-α and IFN-γ) by DCs. (A-B) TLR7/8 and TLR9 agonist was used to stimulate DCs at baseline and after 24 week of mono-therapy and sequential therapy. Mono-therapy showed no significant increase in the production of IFN-α and TNF-α secretion; however, there was significant increase in IFN-α and TNF-α secretion with sequential therapy. (C) Relative expression of miR 155, 146, 221 and 222 in DCs at baseline and after 24 week of mono-therapy and sequential therapy; miR-155 and miR-221 expression in DCs was increased in sequential therapy.