Figure 2: All immunoassay methods involved a 2-step 1 immunoassay with 1hr incubation of CA215, cIgG, or hIgG at 37°C, followed by 1hr incubation at 37°C of the detecting antibody. In the RP215/RP215-HRP immunoassay, wells were coated with 20 μg/mL of RP215 and the detecting antibody used was HRP labeled RP215. In the RP215/GαhIgG-Fc-ALP immunoassay, wells were coated with 20 μg/mL of RP215 and the detecting antibody used was ALP-labeled goat-anti-human IgG. In the GαhIgG/GαhIgG-Fc- ALP immunoassay, wells were coated with 1 μg/mL GαhIgG and the detecting antibody used was ALP-labeled goat-anti-human IgG. Standard errors are presented in each case of the immunoassays. Relative activities are presented using a log scale.