Figure 4: Characteristics of HA+ and HA- Tregs of healthy individuals and breast cancer patients. CD4+ cells were purified by paramagnetic bead cell separation from peripheral blood of 7 healthy donors and 12 breast cancer patients, fractioned into HA+ and HA- and stimulated with TSI for 18 h. CD4+FoxP3+ and CD4+CD25+FoxP3+ Tregs were evaluated for the frequency of CTLA-4+ cells (A) and TGF-β+, IL-35+, LAP+, IL-10+, GITR+ cells (B).