Figure 2:Cyclo (His-Pro) protects hSOD1G93A microglial cells against PQ-induced oxidative stress. hSOD1G93A microglial cells were treated as described. (A) GSH levels (control GSH: 23 ± 5.1 nmoles/mg protein). (2h: F3,59=11.18, P=0.01; 4h: F3,59=18.13, P=0.001; 6h: F3,59=24.89, P=0.001; 24h: F3,59=38.2, P< 0.001 twoway ANOVA, n=3). Data represent mean ± S.D.* vs. untreated cells. # vs. PQ-treated cells. (B) Detection of ROS generation by DCFH-DA fluorescence (100% control ROS fluorescence: 6h: 0.98 ± 0.07; 24h:1.15 ± 0.15). (6h: F3,59=24.04, P=0.001; 24h: F3,59=51,44, P< 0.001 two-way ANOVA, n=3). Data represent mean ± S.D. *vs. untreated cells. #vs. PQ-treated cells.