Figure 2: Heparanase activity. Fluids were freshly collected from chronic empyema (CE) patients or patients with simple paraneumonic effusion (SPE); Fluids were centrifuged (300 g, 10 min), and the supernatants and cell pellets were recovered and evaluated for heparanase enzymatic activity as described under 'Materials and Methods'. Shown are typical heparanase activities (i.e., release of HS degradation fragments from sulfate labeled ECM) in the clear fluids (A) and cell lysates (B).