Figure 2: Proliferation of RAW 264.7 and Maf-DKO cells in the presence of glycolysis inhibitor 2-DG and OXPHOS inhibitor oligomycin. RAW 264.7 (A) and Maf-DKO (B) cells were incubated in control medium or medium containing 10mM 2-DG or 2.5 μM oligomycin for 24, 48, or 72 hours, after which protein content was determined with the SRB assay as described in the legend of Figure 1, and in M&M. In order to enable comparison of proliferation rates, absorbance values were normalized to the control values at 24 hours, since RAW 264.7 and Maf-DKO cells were not seeded at equal densities and absorbance values at 24 hours varied between cell lines. Data represent means ± SEM of three independent experiments performed in triplicate. (**p < 0.01, ***p < 0.001, Twoway ANOVA and Bonferroni posttest)